Aside from playing as Vergil throughout the content, Vergil's Downfall features additional hidden items to collect, two new enemies and a new boss, and has a leaderboard with which to compete with others. The content also comes with four difficulty levels, new combos, and new locations.

Vergil uses many of his traditional skills, including several based on Slash Dimension, Air Trick, and Summoned Swords, and is able to use Devil Trigger to generate a doppelganger.